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Genetics

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用于分离共转录RNA处理事件的人工RNA聚合酶II伸长复合物
 
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用于分离共转录RNA处理事件的人工RNA聚合酶II伸长复合物

Article DOI: 10.3791/59497
May 13th, 2019

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Summary May 13th, 2019

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在这里,我们描述了RNA聚合酶II(Pol II)伸长复合物的组装,只需要短的合成DNA和RNA寡核苷酸和纯化Pol II。这些复合物可用于研究与 Pol II 伸长复合体相关的转录记录的基本机制。

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