JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Genetics
/
RNA足迹的识别:通过RNA免疫沉淀在串联中的蛋白质复合物,然后测序(RIPiT-Seq)
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Genetics
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
Please note that all translations are automatically generated.
Click here for the English version.
RNA足迹的识别:通过RNA免疫沉淀在串联中的蛋白质复合物,然后测序(RIPiT-Seq)
DOI:
10.3791/59913-v
•
09:26 min
•
July 10, 2019
•
Lauren Woodward
,
Pooja Gangras
,
Guramrit Singh
1
Department of Molecular Genetics, Center for RNA Biology
,
The Ohio State University
Chapters
00:04
Title
01:40
Cell Harvesting and Formaldehyde Treatment
02:50
FLAG Immunoprecipitation and RNase I Digestion
04:09
Affinity Elution and Magnetic Bead-antibody Conjugation
05:14
Second Immunoprecipitation
06:12
RNA Extraction and Analysis
07:51
Results: RNA and Protein Yields
08:38
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们提出一个协议,以丰富内源RNA结合位点或"足迹"的RNA:蛋白质(RNP)复合物从哺乳动物细胞。这种方法涉及RNP亚单位的两个免疫沉淀,因此被称为RNA免疫沉淀串联(RIPiT)。
Tags
RNA Immunoprecipitation
RIPiT-Seq
RNA-protein Complexes
Epitope-tagged Protein
CRISPR
Immunoprecipitation
RNA Extraction
HEK293 Cells
RNA Binding Proteins
RNA Processing
Diseases
Neuropathies
Cancer
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
核密度分析的天然染色质沉淀测序文库的生成
用 5-Bromouridine 标记和免疫沉淀 RNA 合成的研究
基于纠错 DNA 和 RNA 测序的稀有事件检测方法
用于分离共转录RNA处理事件的人工RNA聚合酶II伸长复合物
人类胰腺胰岛的单细胞RNA测序与分析
使用RNA测序识别循环RNA
番茄根转化后与
拉子托尼亚索拉纳卡鲁姆
接种,直接遗传分析细菌性威尔特病
植物病原体分泌效应器中RNA沉默抑制器的筛选和鉴定
通过旗-生物素串联纯化通过交叉链接和免疫沉淀对蛋白质-RNA相互作用进行转录组-全过程分析
使用染色质免疫沉淀和高通量测序(ChIP-seq)发现
沙门氏菌
生物膜中的CsgD监管目标
Read Article