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JoVE Journal
Biochemistry

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丝状真菌曲霉菌的单步富集蛋白脱乙酰化酶的酶活性测定
 
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丝状真菌曲霉菌的单步富集蛋白脱乙酰化酶的酶活性测定

Article DOI: 10.3791/59527
May 1st, 2019

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Summary May 1st, 2019

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Click here for the English version.

像 RpdA 这样的1级组蛋白去乙酰化酶 (Hdac) 作为治疗真菌感染的潜在靶点变得越来越重要。在这里, 我们提出了一个协议的特普标签 RpdA 结合 HDAC 活性检测, 允许体外疗效测试组蛋白去乙酰化酶抑制剂的方案.

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