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Biochemistry
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从高梁叶组织中分离出希石,用于自上而下质量光谱分析潜在的表观遗传标记
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
Please note that all translations are automatically generated.
Click here for the English version.
从高梁叶组织中分离出希石,用于自上而下质量光谱分析潜在的表观遗传标记
DOI:
10.3791/61707-v
•
07:10 min
•
March 04, 2021
•
Mowei Zhou
,
Shadan H. Abdali
,
David Dilworth
,
Lifeng Liu
,
Benjamin Cole
,
Neha Malhan
,
Amir H. Ahkami
,
Tanya E. Winkler
,
Joy Hollingsworth
,
Julie Sievert
,
Jeff Dahlberg
,
Robert Hutmacher
5
,
Mary Madera
,
Judith A. Owiti
,
Kim K. Hixson
,
Peggy G. Lemaux
,
Christer Jansson
,
Ljiljana Paša-Tolić
1
Environmental Molecular Sciences Laboratory
,
Pacific Northwest National Laboratory
,
2
DOE-Joint Genome Institute
,
Lawrence Berkeley Laboratory
,
3
Kearney Agricultural Research and Extension Center
,
University of California Agriculture and Natural Resources
,
4
West Side Research and Extension Center
,
University of California
,
5
Department of Plant Sciences
,
University of California, Davis
,
6
Department of Plant and Microbial Biology
,
University of California, Berkeley
Chapters
00:05
Introduction
00:40
Nuclei Isolation from Sorghum Leaf Sample
02:13
Isolation of Nuclei from Remaining Cytoplasmic Contaminants
03:05
Nuclei Lysis and Histone Extraction
04:39
Results: Qualitative & Quantitative Analysis of the Identified Histones
06:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案的制定是为了有效地从高梁叶材料中提取完整的组织结石,用于分析结石转化后的修饰,这些修饰可以作为潜在的表观遗传标记,以帮助工程抗旱作物。
Tags
Histone Extraction
Sorghum
Mass Spectrometry
Epigenetic Markers
Post-translational Modifications
Protease Inhibitors
Nuclei Lysis Buffer
Centrifugation
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