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Generation av Organoids från mus extrahepatiska gallgångarna
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Generation av Organoids från mus extrahepatiska gallgångarna

Article DOI: 10.3791/59544-v • 09:13 min • April 23rd, 2019
April 23rd, 2019 •
1, 1, 1,2, 1,2, 1
1Departments of Internal Medicine, The University of Michigan, 2Molecular & Integrative Physiology, The University of Michigan

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Det här protokollet beskriver produktionen av en mus extrahepatiska gallvägar 3-dimensionell organoid system. Dessa biliär organoids kan upprätthållas i kulturen att studera cholangiocyte biologi. Gallan organoids express markörer både stamceller och biliär celler och består av polariserade epitelceller.

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Utvecklingsbiologi fråga 146 biliär progenitorceller cholangiocytes organoids 3-dimensionell kultur system drogkontroll cholangiopathies
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