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Bioengineering

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JoVE Journal Bioengineering
Preparazione di nanoparticelle polimeriche neutralmente cariche e sensibili al pH per la consegna del siRNA citosolico
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Journal (Bioengineering)
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Journal (Bioengineering)
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Preparazione di nanoparticelle polimeriche neutralmente cariche e sensibili al pH per la consegna del siRNA citosolico

Article DOI: 10.3791/59549-v • 09:09 min • May 2nd, 2019
May 2nd, 2019 •
1, 1, 1,2,3
1Department of Chemical Engineering, University of Mississippi, 2Department of BioMolecular Sciences, University of Mississippi, 3Biomedical Engineering Program, University of Mississippi

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Sono presentati metodi per preparare e caratterizzare le proprietà fisico-chimiche e la bioattività delle nanoparticelle siRNA reattive al pH neutralmente caricate. Sono discussi i criteri per le nanomedicine siRNA di successo come le dimensioni, la morfologia, la carica superficiale, il carico di siRNA e il silenziamento genico.

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Bioingegneria problema 147 interferenza dell'RNA fuga endosomiale Endosomolisi reattività del pH nanotecnologia nanoparticelle polimeriche ingegneria biomedica somministrazione di farmaci cancro
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