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Fremstilling af Neutrally-ladede, pH-Responsive polymere nanopartikler til Cytosolic siRNA-levering
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Bioengineering
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JoVE Journal Bioengineering
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Fremstilling af Neutrally-ladede, pH-Responsive polymere nanopartikler til Cytosolic siRNA-levering

DOI:
10.3791/59549-v

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09:09 min

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May 02, 2019

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, ,
1Department of Chemical Engineering,University of Mississippi, 2Department of BioMolecular Sciences,University of Mississippi, 3Biomedical Engineering Program,University of Mississippi

Chapters

  • 00:04Title
  • 00:49Preparation and Characterization of si-NPs (siRNA Nanoparticles)
  • 02:00Physicochemical Characterization of si-NPs
  • 05:04Determining in vitro Bioactivity of si-NPs
  • 06:35Results: Comparison between si-NP 1 and si-NP 2 and siRNA Complexation
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

Metoder til at forberede og karakterisere de fysisk-kemiske egenskaber og Bioaktivitet af neutrally-ladede, pH-Responsive siRNA nanopartikler præsenteres. Kriterier for vellykkede siRNA nanomediciner såsom størrelse, morfologi, overflade ladning, siRNA lastning, og genhæmning diskuteres.

Tags

SiRNANanoparticlesCytosolic DeliveryPH-responsiveNeutrally-chargedDynamic Light ScatteringTransmission Electron MicroscopySystemic AdministrationBioavailability

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