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JoVE Journal Cancer Research
Extraction de métabolites aqueux de cellules adhérentes cultivées pour analyse métabolomique par électrophorèse capillaire-spectrométrie de masse
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Extraction de métabolites aqueux de cellules adhérentes cultivées pour analyse métabolomique par électrophorèse capillaire-spectrométrie de masse

Article DOI: 10.3791/59551-v • 11:39 min • June 9th, 2019
June 9th, 2019 •
1,2, 3, 3, 3, 1,2, 4, 1,2,4
1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory, National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center

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Le but de cet article est de décrire un protocole pour l’extraction de métabolites aqueux de cellules adhérentes cultivées pour l’analyse métabolomique, en particulier, l’électrophorèse capillaire-spectrométrie de masse.

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Recherche sur le cancer numéro 148 métabolome cellules cancéreuses électrophorèse capillaire-spectrométrie de masse (CE-MS) glucose-6-phosphate déshydrogénase (G6PD) stress oxydatif diamide
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