Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Lentiviral mediato gene silenziamento in…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
Lentiviral mediato gene silenziamento in pseudoislet umano preparato in piastre a basso attaccamento
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
Journal (Genetics)
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
Journal (Genetics)
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
 
Click here for the English version

Lentiviral mediato gene silenziamento in pseudoislet umano preparato in piastre a basso attaccamento

Article DOI: 10.3791/59578-v • 11:24 min • May 14th, 2019
May 14th, 2019 •
1,2, 1,2, 1,2, 2,3, 2,3, 1,2
1Department of Internal Medicine, Carver College of Medicine, University of Iowa, 2Fraternal Order of Eagles Diabetes Research Center, University of Iowa, 3Roy J. Carver Department of Biomedical Engineering, University of Iowa

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Viene presentato un protocollo per creare pseudoislets umani modificati in gene da cellule di isole umane disperse che vengono tradusse da lentivirus che trasportano RNA a forcina corta (shRNA). Questo protocollo utilizza vasi enzimatici e di coltura prontamente disponibili, può essere eseguito facilmente e produce pseudoislets umani geneticamente modificati adatti per studi funzionali e morfologici.

Tags

Genetica Numero 147 shRNA lentivirus secrezione di insulina coltura singola cellula riaggregazione
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter