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JoVE Journal Neuroscience
Quantificando a ubiquitina subcelular-atividade de proteasome no cérebro de roedores
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Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
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Quantificando a ubiquitina subcelular-atividade de proteasome no cérebro de roedores

Article DOI: 10.3791/59695-v • 09:25 min • May 21st, 2019
May 21st, 2019 •
1, 2, 1,2
1Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, 2School of Neuroscience, Virginia Polytechnic Institute and State University

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Este protocolo é projetado para quantificar eficientemente a atividade do sistema de ubiquitina-proteasome (UPS) em diferentes compartimentos celulares do cérebro de roedores. Os usuários podem examinar o funcionamento da UPS em frações nucleares, citoplasmáticas e sinápticas no mesmo animal, reduzindo a quantidade de tempo e o número de animais necessários para realizar essas análises complexas.

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Neurociência edição 147 ubiquitina proteasome núcleo sinapse citoplasma memória hipocampo amígdala
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