Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

Dmitry Frank; Benjamin  F. Gruenbaum; Julia Grinshpun; Israel Melamed; Olena Severynovska; Ruslan Kuts; Michael Semyonov; Evgeni Brotfain; Alexander Zlotnik; Matthew Boyko

doi:10.3791/61309

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Medição de edema cerebral pós-acidente vascular cerebral, zona de infarto e quebra da barreira hematoencefálica em um único conjunto de amostras cerebrais de roedores

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

Medição de edema cerebral pós-acidente vascular cerebral, zona de infarto e quebra da barreira hematoencefálica em um único conjunto de amostras cerebrais de roedores

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7,812 Views

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04:32 min

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October 23, 2020

DOI:

10.3791/61309-v

DOI:

10.3791/61309-v

•

04:32 min

October 23, 2020

•

5 Views

Dmitry Frank*¹, Benjamin F. Gruenbaum*², Julia Grinshpun¹, Israel Melamed³, Olena Severynovska⁴, Ruslan Kuts¹, Michael Semyonov¹, Evgeni Brotfain¹, Alexander Zlotnik¹, Matthew Boyko¹

¹Department of Anesthesiology and Critical Care, Soroka Medical Center,Ben-Gurion University of the Negev, ²Department of Anesthesiology,Yale University School of Medicine, ³Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences,Ben-Gurion University of the Negev, ⁴Department of Physiology, Faculty of Biology, Ecology and Medicine,Dnepropetrovsk State University

Transcript

Summary

Este protocolo descreve uma nova técnica de medição dos três parâmetros mais importantes de lesão cerebral isquêmica no mesmo conjunto de amostras cerebrais de roedores. Usar apenas uma amostra cerebral é altamente vantajoso em termos de custos éticos e econômicos.