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Cancer Research
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评估癌细胞3D球形培养中的细胞活力和死亡
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Cancer Research
Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
Please note that all translations are automatically generated.
Click here for the English version.
评估癌细胞3D球形培养中的细胞活力和死亡
DOI:
10.3791/59714-v
•
10:33 min
•
June 16, 2019
•
Michala G. Rolver
,
Line O. Elingaard-Larsen
,
Stine F. Pedersen
1
Section for Cell Biology and Physiology, Department of Biology, Faculty of Science
,
University of Copenhagen
Chapters
00:04
Title
00:59
Spontaneous Spheroid Formation
01:45
Reconstituted Basement Membrane-mediated Spheroid Formation
03:04
Hanging Drop Spheroids
04:14
Preparing Protein Lysates for Western Blotting from 3D Spheroid Cultures
05:21
Propidium Iodide (PI) Staining of Spheroids
06:27
Embedding of Spheroids
07:52
Results: Assessing Cell Viability and Death
09:24
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了几种简单的方法来评估3D癌细胞球体的生存能力和死亡,它模仿体内肿瘤的物理化学梯度比2D培养要好得多。因此,球形模型允许评估癌症药物疗效,并改进对体内条件的翻译。
Tags
3D Spheroid Culture
Cell Viability
Cell Death
Cancer Cells
Tumor Microenvironment
Drug Screening
Immunohistochemistry
Basement Membrane
Cell Suspension
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