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Immunology and Infection
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Quantification of Atherosclerosis in Mice
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of Atherosclerosis in Mice

Quantification of Atherosclerosis in Mice

DOI:
10.3791/59828-v

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06:59 min

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June 12, 2019

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, , ,
1Cardiovascular Medicine Unit, Center for Molecular Medicine, Department of Medicine,Karolinska Institutet, Karolinska University Hospital, 2Department of Cardiovascular and Renal Research, Institute for Molecular Medicine,University of Southern Denmark (SDU)

Chapters

  • 00:04Title
  • 00:56Murine Aorta Microdissection
  • 01:57En Face Aortic Arch and Brachiocephalic Artery Analysis
  • 04:20Aortic Root Cryosectioning
  • 05:29Results: Representative Atherosclerotic Lesion Analyses
  • 06:31Conclusion

Summary

Automatic Translation

Automatic Translation

Murine models of atherosclerosis are useful tools to investigate pathogenic pathways on a molecular level, but require standardized quantification of lesion development. This protocol describes an optimized method to determine lesion size in the major arterial vessels including the aortic root, aortic arch, and brachiocephalic artery.

Tags

Keywords: AtherosclerosisMouse ModelsCardiovascular DiseaseAortic RootAortic ArchBrachiocephalic ArteryLesion Size QuantificationCryosectioningEn Face AnalysisAortaMicroscopyDissection

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