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Journal / Developmental Biology / キトサン膜上のヒト歯周靭帯細胞スフェロイドの形成…
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Developmental Biology

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JoVE Journal Developmental Biology
キトサン膜上のヒト歯周靭帯細胞スフェロイドの形成
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キトサン膜上のヒト歯周靭帯細胞スフェロイドの形成

Article DOI: 10.3791/59855-v • 04:14 min • June 19th, 2019
June 19th, 2019 •
*1, *2, 1, 1, 1, 3, 1
1Department of Periodontology, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 2Department of Periodontology, Dental Diseases Prevention & Treatment Center of Jiading District, 3College of Materials Science and Engineering, Tongji University
* These authors contributed equally

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ここでは、ヒト歯周靭帯(PDL)細胞スフェロイドをキトサン膜で培養するプロトコルを紹介する。三次元(3D)細胞スフェロイドの培養は、従来の組織培養ポリスチレン(TCPS)培養システムに代わるものとなる。

Tags

発生生物学,第148号,スフェロイド,三次元,細胞培養,歯周靭帯細胞,キトサンフィルム,歯周再生
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