A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences, 2State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences,Peking University
Chapters
- 00:04Title
- 01:15Installation and Testing
- 02:56Identifying Novel MicroRNAs (miRNAs)
- 03:33Reference Index Building
- 04:42Results: Representative miRDP2 Performance
- 05:45Conclusion
Un pipeline bioinformatique, à savoir miRDeep-P2 (miRDP2 pour faire court), avec des critères de miARN végétal mis à jour et un algorithme révisé, pourrait analyser avec précision et efficacité les transcriptomes de microARN chez les plantes, en particulier pour les espèces ayant des génomes complexes et volumineux.
Tags
Bioinformatics PipelineMicroRNA TranscriptomesPlantsMiRDeep2Plant MicroRNAsTranscriptional RegulatorsPlant DevelopmentEnvironmental ChallengesFalse PositivesProcessing TimeHigh Confidence MicroRNA AnnotationGenome FunctionMiRDeep2 PipelineArabidopsis ThalianaGenome Reference IndexNoncoding RNA Reference IndexDeep Sequencing ReadsAdaptersFASTA Identifiers
