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Genetics

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Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
 

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Article doi: 10.3791/59104
March 24th, 2019

Summary March 24th, 2019

This protocol outlines experimental procedures to characterize genome-wide changes in the levels of histone post-translational modifications (PTM) occurring in connection with the overexpression of proteins associated with ALS and Parkinson's disease in Saccharomyces cerevisiae models. After SDS-PAGE separation, individual histone PTM levels are detected with modification-specific antibodies via Western blotting.

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