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Optimering, design og undgåelse af faldgruber i manuel multiplex fluorescerende immun histokemi
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Optimering, design og undgåelse af faldgruber i manuel multiplex fluorescerende immun histokemi

Article DOI: 10.3791/59915-v • 09:15 min • July 26th, 2019
July 26th, 2019 •
1, 1, 1, 1, 1, 2, 3, 2, 1, 1, 1
1Department of Surgery, University of Michigan, 2Department of Molecular and Cellular Physiology, University of Michigan, 3Department of Pathology, University of Michigan

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Multiplex fluorescerende Immunhistokemi er en ny teknologi, der muliggør visualisering af flere celletyper inden for intakt formalin-fast, paraffinindlejret (FFPE) væv. Præsenteret er retningslinjer for at sikre en vellykket 7-farve multiplex med instruktioner til optimering af antistoffer og reagenser, forberede dias, design og tips til at undgå almindeligt problemer.

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Kræftforskning kræft metastase immunofluorescens multiplex fluorescerende immunhistokemi mikromiljø celle-til-celle engagement farvning fluoroforet
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