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I Vivo Målretning af Xenografted humane kræftceller med funktionaliserede fluorescerende Silica nanopartikler i zebrafisk
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1Departments of Pharmacology and Medicine, Section of Hematology and Medical Oncology, The Cancer Research Center,Boston University School of Medicine, 2Department of Biomedical Engineering,Tufts University, 3Department of Mechanical Engineering,Tufts University, 4Department of Physics,Tufts University
Chapters
- 00:04Introduction
- 00:54Generation of Casper Zebrafish Embryos
- 02:33Preparing Materials for Cancer Cell Transplantation
- 03:34Preparing Cancer Cells for Transplantation
- 05:20Transplantation of Cancer Cells
- 06:54Injection of Nanoparticles or Vehicle
- 07:40Imaging and Tracking of Nanoparticles and Cancer Cells
- 08:45Results: Colocalization of Cancer Cells and Nanoparticles
- 09:47Conclusion
Beskrevet her er en metode til at udnytte zebrafisk embryoner til at studere evne til funktionaliserede nanopartikler til at målrette menneskelige kræftceller in vivo. Denne metode gør det muligt at evaluere og vælge optimale nanopartikler til fremtidige forsøg med store dyr og i kliniske forsøg.
