JoVE Journal
Genetics
Genetics
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Chapters
Summary
This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.
Tags
Frozen Tissue Comet Assay DNA Damage Evaluation Logistics Remote Laboratory Tissue Analysis Personnel Training Technical Errors Mechanical DNA Damage Pre-clinical Drug Development Safety Assessment Environmental Bio-monitoring Genotoxic Potential DNA Repair Protective Effects Chemotherapy Radiation Food Additives
PLAYLIST
The Theory of Receptors and Ligands
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Education • Core: BiologyG-protein Coupled Receptors -
Education • Core: BiologyInternal Receptors -
Education • Core: BiologyWhat are Second Messengers? -
Education • Core: BiologyIntracellular Signaling Cascades -
Education • Core: BiologyInduced-fit Model -
Education • Core: BiologyEnzyme Inhibition -
Education • Core: BiologyAllosteric Regulation -
Education • Core: BiologyCofactors and Coenzymes -
Research • Immunology and InfectionCo-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
Basic Research Tools
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Education • Biomedical EngineeringImaging Biological Samples with Optical and Confocal Microscopy -
Education • Basic Methods in Cellular and Molecular BiologyAn Introduction to Transfection -
Education • Cell BiologyThe Transwell Migration Assay -
Research • Bioengineering2D and 3D Matrices to Study Linear Invadosome Formation and Activity -
Research • BiologyAnalysis of Cell Cycle Position in Mammalian Cells -
Education • Cell BiologyCell-surface Biotinylation Assay -
Education • Cell BiologyThe ATP Bioluminescence Assay -
Education • Cell BiologyDetecting Reactive Oxygen Species -
Education • Cell BiologyThe TUNEL Assay -
Research • Cancer ResearchAssessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells -
Research • BiologyViability Assays for Cells in Culture
Cell-Based Assays
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Research • NeuroscienceMethods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission -
Research • Cancer ResearchDetecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay -
Research • MedicineA Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators -
Research • BiologyA BW Reporter System for Studying Receptor-Ligand Interactions -
Research • MedicineAn Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells -
Research • BiochemistryAn Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects -
Research • BioengineeringTherapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
Tissue-Based Testing
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Research • MedicineBladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology -
Research • BiologyMeasurement of Smooth Muscle Function in the Isolated Tissue Bath-applications to Pharmacology Research -
Research • Immunology and InfectionThe Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids -
Research • MedicineForskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients -
Research • NeuroscienceProbing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine -
Research • BioengineeringAutomated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening -
Research • NeuroscienceA Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain -
Research • ChemistryAn Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
In Vivo Testing
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Research • MedicineLive Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer -
Research • Immunology and InfectionA Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii -
Research • NeuroscienceSystemic and Local Drug Delivery for Treating Diseases of the Central Nervous System in Rodent Models -
Research • BiologyA Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection -
Research • Immunology and InfectionThe Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport -
Research • MedicineUse of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs -
Research • MedicinePercutaneous Contrast Echocardiography-guided Intramyocardial Injection and Cell Delivery in a Large Preclinical Model -
Research • MedicineA Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: Application to Drug Efficacy Studies
Dose-Response Curves
Drug Screening
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Research • BiochemistryKinase Inhibitor Screening In Self-assembled Human Protein Microarrays -
Research • BiologyAvidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions -
Research • BiologyHigh-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels -
Research • BiochemistryParallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay -
Research • Immunology and InfectionIdentification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries -
Research • Cancer ResearchPhysiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells -
Research • Bioengineering3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
Assessing Genotoxicity
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Research • Cancer ResearchEvaluating In Vitro DNA Damage Using Comet Assay -
Research • GeneticsUse of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage -
Research • BiologyRapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae -
Research • NeuroscienceAssessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress -
Research • BioengineeringAdvanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure -
Research • MedicineGene-environment Interaction Models to Unmask Susceptibility Mechanisms in Parkinson's Disease
Toxicology Testing
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Research • BiologyHigh Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC) -
Research • BiologyExperimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory -
Research • Developmental BiologyHuman Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation -
Research • BiologyDetecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory -
Research • Developmental BiologyProtocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
