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使用CRISPR/Cas9系统将基因挖空直接引入锥虫瘤的肌瘤阶段
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JoVE Journal Genetics
Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System
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使用CRISPR/Cas9系统将基因挖空直接引入锥虫瘤的肌瘤阶段

DOI:
10.3791/59962-v

•

06:13 min

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July 31, 2019

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, , ,
1Biomedical Research Institute,National Institute of Advanced Industrial Science and Technology (AIST)

Chapters

  • 00:04Title
  • 00:35Differentiation of Trypomastigotes into Extracellular Amastigotes
  • 01:54Electroporation of Extracellular Amastigotes
  • 03:01Monitoring the Growth of Knockout Cells
  • 04:35Results: Growth Monitoring and Phenotypic Differences of Knockout Parasites
  • 05:41Conclusion

Summary

Automatic Translation

Automatic Translation

在这里,我们描述了一个协议,使用CRISPR/Cas9系统将基因敲除引入锥虫的细胞外肌瘤。生长表型可以通过细胞计数来追踪轴酸乳腺培养物,或者通过宿主细胞入侵后细胞内乳腺增殖。

Tags

Gene KnockoutCRISPR/Cas9Trypanosoma CruziAmastigoteHost Cell InfectionParasitologyExtracellular AmastigotesTrypomastigotesCell CultureElectroporation

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