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Developmental Biology

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JoVE Journal Developmental Biology
En forbedret grøn fluorescens protein-baseret assay til at studere Neurite udvækst i primære neuroner
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En forbedret grøn fluorescens protein-baseret assay til at studere Neurite udvækst i primære neuroner

Article DOI: 10.3791/60031-v • 08:02 min • October 19th, 2019
October 19th, 2019 •
*1, *1,2, *1, 1
1School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 2Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen
* These authors contributed equally

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I denne rapport beskriver vi en simpel protokol til undersøgelse af neurit-vækst i embryonale rotte kortikale neuroner ved co-transftering med EGFP og det protein af interesse.

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Udviklingsmæssige biologi forbedret grønt fluorescerende protein ImageJ mikroskopi neurite outgrowth primære neuroner transfektering
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