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Journal / Genetics / Verbetering van kleine RNA-SEQ: minder bias en…
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JoVE Journal Genetics
Verbetering van kleine RNA-SEQ
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Verbetering van kleine RNA-SEQ: minder bias en betere detectie van 2 '-O-methyl Rna's

Article DOI: 10.3791/60056-v • 08:49 min • September 16th, 2019
September 16th, 2019 •
1, 1, 1
1Institute for Integrative Biology of the Cell, UMR9198, CNRS CEA Univ Paris-Sud, Université Paris-Saclay

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We presenteren een gedetailleerd klein RNA Library Reparatie protocol met minder bias dan standaardmethodes en een verhoogde gevoeligheid voor 2 '-O-methyl rna's. Dit protocol kan worden gevolgd met behulp van zelfgemaakte reagentia om kosten te besparen of kits te gebruiken voor het gemak.

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Genetica uitgave 151 klein RNA kleine RNA-seq bias bibliotheek voorbereiding sequentiëren van de volgende generatie NGS 2 '-O-methyl (2 '-OMe) RNA plant microRNA plant miRNA
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