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Genetics

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JoVE Journal Genetics
Detectie van post-replicatieve hiaten accumulatie en reparatie in menselijke cellen met behulp van de DNA-vezeltest
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Detectie van post-replicatieve hiaten accumulatie en reparatie in menselijke cellen met behulp van de DNA-vezeltest

Article DOI: 10.3791/63448-v • 10:32 min • February 3rd, 2022
February 3rd, 2022 •
*1, *2, 2, 1
1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, 2Division of Oncology, Department of Internal Medicine, Washington University in St. Louis
* These authors contributed equally

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Hier beschrijven we twee modificaties van de DNA-vezeltest om enkelstrengs DNA-hiaten te onderzoeken bij het repliceren van DNA na laesie-inductie. De S1-vezeltest maakt de detectie van post-replicatieve hiaten mogelijk met behulp van het ssDNA-specifieke S1-endonuclease, terwijl de gap-filling assay visualisatie en kwantificering van gap repair mogelijk maakt.

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Genetica post-replicative single-stranded DNA gap gap-filling post replication repair DNA replication DNA fiber assay S1 nuclease repriming
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