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Differensiering av mus bryst epitel HC11 og EpH4 celler
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Differensiering av mus bryst epitel HC11 og EpH4 celler

Article DOI: 10.3791/60147-v • 09:32 min • February 27th, 2020
February 27th, 2020 •
*1,2, *1, *1,3, 1,4, 1, 1, 2, 1
1Department of Biomedical and Molecular Sciences and Department of Pathology and Molecular Medicine, Queen's University, Kingston, 2Department of Chemical and Physical Sciences, University of Toronto, Mississauga, 3Department of Laboratory Medicine, Brampton Civic Hospital Campus, William Osler Health System, 4Latner Thoracic Surgery Research Laboratories
* These authors contributed equally

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Vi beskriver teknikker for differensiering induksjon av to bryst epitellinjer, HC11 og EpH4. Mens begge krever fosterkalvserum, insulin og prolaktin for å produsere melkeproteiner, kan EpH4-celler fullt ut skille seg inn i mammosfærer i tredimensjonal kultur. Disse komplementære modellene er nyttige for signaltransduksjonsstudier av differensiering og neoplasi.

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Kreftforskning Utgave 156 celledifferensiering ammeepitelceller prolaktin EHS-matrise
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