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使用分裂的角质按钮的孔膜内皮器官培养模型
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Medicine
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JoVE Journal
Medicine
A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons
Please note that all translations are automatically generated.
Click here for the English version.
使用分裂的角质按钮的孔膜内皮器官培养模型
DOI:
10.3791/60171-v
•
08:36 min
•
October 06, 2019
•
Daniel A. Wenzel
,
Berenike C. Kunzmann
,
Nils A. Steinhorst
,
Martin S. Spitzer
,
Maximilian Schultheiss
1
Department of Ophthalmology
,
University Medical Center Hamburg-Eppendorf (UKE)
,
2
University Eye Hospital, Center For Ophthalmology
,
University Hospital Tübingen
Chapters
00:04
Title
00:55
Porcine Eye Preparation
01:35
Dissection and Incubation
03:43
Microscopy and Endothelium Examination
05:11
Corneal Endothelium Image Processing
06:15
Corneal Endothelial Cell Density and Morphological Parameter Analysis
06:49
Results: Representative Corneal Button Culture Analyses
08:12
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里提出了猪分裂角膜纽扣的制备和培养的分步方案。由于这种通常培养的器官培养模型显示细胞死亡率在15天内,相当于人类捐赠角膜,它代表了第一个模型,允许长期种植非人类角膜,而不添加有毒的dextran。
Tags
Porcine Corneal Endothelial Organ Culture
Split Corneal Buttons
Corneal Endothelial Cell Cultivation
Disinfection
Fetal Calf Serum
Dextran-free Culture Medium
Eye Bulb Holder
Corneal Trephination
Suture
Anterior Eye Chamber
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