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Neuroscience
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发展
果蝇
神经元的快速丹德林分体动力学的延时实时成像和定量
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing
Drosophila
Neurons
Please note that all translations are automatically generated.
Click here for the English version.
发展
果蝇
神经元的快速丹德林分体动力学的延时实时成像和定量
DOI:
10.3791/60287-v
•
08:23 min
•
September 25, 2019
•
Chengyu Sheng
,
Uzma Javed
,
Justin Rosenthal
,
Jun Yin
,
Bo Qin
,
Quan Yuan
1
National Institute of Neurological Disorders and Stroke
,
National Institutes of Health
Chapters
00:04
Title
00:54
Larval Brain Explant Dissection and Mounting
02:04
Live Time-Lapse Imaging
03:02
Drift Correction and Deconvolution
03:54
Image Annotation
05:06
Dynamic Dendritic Branch Calculation
06:43
Results: Representative Dynamic Dendritic Branch Terminal Tracking
07:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了我们用于在
果蝇
幼虫脑活制备中成像高度多瓣树突状丝虫的方法,以及我们为量化延时三维成像数据集用于对树突进行定量评估的协议发展神经元的动态。
Tags
Time-lapse Imaging
Dendritic Branch Dynamics
Drosophila Neurons
3D Image Annotation
Branch Terminal Tracking
Dendrite Morphogenesis
Larval Brain Dissection
External Saline Solution
Two-photon Microscopy
Drift Correction
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