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Journal / Immunology and Infection / Infezione da pneumococco delle cellule primarie…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Infezione da pneumococco delle cellule primarie endoteliali nel flusso costante
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Infezione da pneumococco delle cellule primarie endoteliali nel flusso costante

Article DOI: 10.3791/60323-v • 09:34 min • October 31st, 2019
October 31st, 2019 •
1,2, 1,3, 1,4, 1
1Institut für Mikrobiologie, Technische Universität Braunschweig, 2Devision of Infection Medicine, Department of Clinical Science, Lund University, 3Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Ludwig Maximilians University, 4Department of Molecular Infection Biology, Helmholtz Center for Infection Research

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Questo studio descrive il monitoraggio microscopico dell'aderenza allo pneumococco alle stringhe del fattore von Willebrand prodotte sulla superficie di cellule endoteliali primarie umane differenziate sotto stress da taglio in condizioni di flusso definite. Questo protocollo può essere esteso alla visualizzazione dettagliata di specifiche strutture cellulari e alla quantificazione dei batteri applicando procedure di immunostaining differenziale.

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Immunologia e Infezione Numero 152 Streptococcus pneumoniae cellule microfluidiche endoteliali microscopia fluorescenza stress da taglio aderenza
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