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Uttrykk, rensing, og liposome binding av spirende gjær SNX-BAR heterodimerer
JoVE Journal
Genetics
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JoVE Journal Genetics
Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
DOI:

10:28 min

December 06, 2019

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Chapters

  • 00:04Title
  • 00:58Yeast Induction and Sorting Nexin-Bin-Amphiphysin-Rvs (SNX-BAR) Dimer Purification
  • 03:52Liposome Preparation
  • 06:44SNX-BAR Liposome Binding and Tubulation
  • 08:26Results: Representative SNX-BAR Induction Verification, Purification, and Liposome Binding and Tubulation Assays
  • 09:38Conclusion

Summary

Automatic Translation

Her presenterer vi en arbeidsflyt for uttrykk, rensing og liposome binding av SNX-BAR heterodimerer i gjær.

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