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Genetics

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Production and Purification of Baculovirus for Gene Therapy Application
 

Production and Purification of Baculovirus for Gene Therapy Application

Article DOI: 10.3791/57019 06:11 min April 9th, 2018
April 9th, 2018

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Summary

In this protocol, baculovirus is produced by transient transfection of baculovirus plasmid into Sf9 cells and amplified in a serum-free suspension culture. The supernatant is purified by heparin affinity chromatography and further concentrated by ultracentrifugation. This protocol is useful for the production and purification of baculovirus for gene therapy application.

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Tags

Production Purification Baculovirus Gene Therapy Insect Cell Culture Heparin Affinity Chromatography Concentration In Vivo Gene Transfer Vaccine Toxicity Inflammation Immune Response Suspension Culture Sf9 Cells Orbital Shaker Incubator P2 Baculovirus Stock Cytopathic Effect Titer Baculovirus Production Amplification
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