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Genetics

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Production and Purification of Baculovirus for Gene Therapy Application
 

Production and Purification of Baculovirus for Gene Therapy Application

Article DOI: 10.3791/57019
April 9th, 2018

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Summary April 9th, 2018

In this protocol, baculovirus is produced by transient transfection of baculovirus plasmid into Sf9 cells and amplified in a serum-free suspension culture. The supernatant is purified by heparin affinity chromatography and further concentrated by ultracentrifugation. This protocol is useful for the production and purification of baculovirus for gene therapy application.

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