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使用Tg（Vtg1：mcherry）斑马鱼胚胎测试内分泌干扰化合物的雌激素效应
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Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds
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使用Tg（Vtg1：mcherry）斑马鱼胚胎测试内分泌干扰化合物的雌激素效应

DOI:
10.3791/60462-v

•

12:02 min

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August 08, 2020

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, , , , , , , , ,
1Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences,Szent István University, 2Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences,University of Birmingham

Chapters

  • 00:00Introduction
  • 02:52Embryon Harvest and Treatment
  • 05:05Larvae Preparation fo Photography
  • 06:05Microscopy
  • 07:02Determining Integrated Density
  • 08:14Representative Results
  • 10:20Conclusions

Summary

Automatic Translation

Automatic Translation

目前这里是使用斑马鱼胚胎Tg（vtg1：mCherry）来检测雌激素效应的详细协议。该协议涵盖鱼类的繁殖和胚胎的治疗，并强调内分泌干扰化合物（EDC）引起的荧光信号的检测、文献和评估。

Tags

Tg(Vtg1:mcherry) ZebrafishEndocrine Disrupting CompoundsEstrogenic EffectsBiomarkerVitellogeninIn Vivo TestingEnvironmental SamplesAnimal Welfare

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