Journal
/
/
使用Tg(Vtg1:mcherry)斑马鱼胚胎测试内分泌干扰化合物的雌激素效应
JoVE Journal
Environment
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds
DOI:

12:02 min

August 08, 2020

, , , , , , , , ,

Chapters

  • 00:00Introduction
  • 02:52Embryon Harvest and Treatment
  • 05:05Larvae Preparation fo Photography
  • 06:05Microscopy
  • 07:02Determining Integrated Density
  • 08:14Representative Results
  • 10:20Conclusions

Summary

Automatic Translation

目前这里是使用斑马鱼胚胎Tg(vtg1:mCherry)来检测雌激素效应的详细协议。该协议涵盖鱼类的繁殖和胚胎的治疗,并强调内分泌干扰化合物(EDC)引起的荧光信号的检测、文献和评估。

Related Videos

Read Article