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Journal
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Immunology and Infection
/
澄清和成像
念珠菌生物
膜
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Clarifying and Imaging
Candida albicans
Biofilms
Please note that all translations are automatically generated.
Click here for the English version.
澄清和成像
念珠菌生物
膜
DOI:
10.3791/60718-v
•
11:09 min
•
March 06, 2020
•
Frederick Lanni
,
Katherine Lagree
,
Manning Y. Huang
,
Lan Yan
,
Carol A. Woolford
,
Aaron P. Mitchell
1
Department of Biological Sciences
,
Carnegie Mellon University
,
2
Department of Pharmacology
,
Second Military Medical University
Chapters
00:04
Introduction
00:48
Growth of Biofilm Cultures
01:34
Specimen Processing for Imaging
02:39
Clarification Protocol: Biofilms on Impermeant Substrata
04:30
Imaging Setup for Confocal Microscopy
05:41
Fast Image Processing and Display for Large Confocal Image Stacks
08:21
Results: Optimum Index Matching and Deep Imaging of Mutant and Wild Type
C. albicans
Biofilms
09:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
为了查看和量化
念珠菌生物
膜的内部特征,我们制备了固定完整的标本,通过折射率匹配进行澄清。然后,利用光学切片显微镜通过生物膜全厚度获取三维图像数据。
Tags
Candida Albicans
Biofilm
Clarification
Optical Sectioning
Confocal Microscopy
Fixation
Staining
Transparency
3D Imaging
Article
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