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Journal / Developmental Biology / Imaging fluorescente del calcio e successiva…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Imaging fluorescente del calcio e successiva ibridazione in situ per la caratterizzazione neuronale del precursore in Xenopus laevis
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Imaging fluorescente del calcio e successiva ibridazione in situ per la caratterizzazione neuronale del precursore in Xenopus laevis

Article DOI: 10.3791/60726-v • 09:07 min • February 18th, 2020
February 18th, 2020 •
1, 2, 3, 4, 4, 5, 5, 2
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard University, 2Department of Biology, College of William and Mary, 3Harvard Medical School, Harvard University, 4Department of Bioengineering, California Institute of Technology, 5Department of Computer Science, College of William and Mary

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Vi presentiamo un protocollo in due parti che combina l'imaging fluorescente del calcio con l'ibridazione in situ, permettendo allo sperimentatore di correlare i modelli di attività del calcio con i profili di espressione genica a livello unicellulare.

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Biologia dello sviluppo Numero 156 Ca2 calcio imaging di calcio attività di calcio sviluppo neurale sviluppo embrionale neurotrasmettitore neuroscienze
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