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高频超声超声心动图评估斑马鱼心脏功能

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Developmental Biology

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JoVE Journal Developmental Biology

High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

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高频超声超声心动图评估斑马鱼心脏功能

DOI:

10.3791/60976-v

•

08:34 min

•

March 12, 2020

•

Alessandro Evangelisti, Katharina Schimmel, Shaurya Joshi, Kavya Shah, Sudeshna Fisch, Kevin M. Alexander, Ronglih Liao, Isabel Morgado

¹Stanford Cardiovascular Institute,Stanford University, ²Division of Genetics, Department of Medicine,Brigham and Women’s Hospital, Harvard Medical School

Chapters

00:05Introduction
00:48Experimental Set-up
02:18Handling the Fish
03:13Image Acquisition and Fish Recovery
05:43Image Analysis
06:31Results: B-Mode Images and Pulse Wave Doppler Images
07:22Conclusion

Summary

Automatic Translation

我们描述了一种使用高频回声心动图评估成年斑马鱼心脏形态和功能的协议。该方法允许心脏可视化和随后定量功能参数，如心率（HR）、心脏输出（CO）、小数面积变化（FAC）、弹出分数（EF）以及血液流入和流出速度。

Tags

Zebrafish Echocardiography Cardiac Function Noninvasive High-resolution Adult Heart Disease Drug Screens Imaging Transducer Anesthesia Micromanipulator

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