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Proteolytisch afgebroken alginaat hydrogels en hydrofobe microbioreactoren voor porcine Oocyte Inkapseling
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Proteolytisch afgebroken alginaat hydrogels en hydrofobe microbioreactoren voor porcine Oocyte Inkapseling

Article DOI: 10.3791/61325-v • 07:45 min • July 30th, 2020
July 30th, 2020 •
1, 2, 3, 1
1Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Krakow, 2Department of Histology, Jagiellonian University Medical College, 3Department of Experimental Hematology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Krakow

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Hier worden twee protocollen gepresenteerd voor de inkapseling van varkensocyten in 3D-kweekomstandigheden. In de eerste worden cumulus-eicellencomplexen (COC's) ingekapseld in fibrin-alginaatkralen. In de tweede zijn ze ingesloten met gefluoreerde ethyleenpropyleenpoederdeeltjes (microbioreactoren). Beide systemen zorgen voor optimale omstandigheden om hun 3D-organisatie te onderhouden.

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Deze maand in JoVE cumulus-eicellen complexen 3D inkapseling alginaat microbioreactoren FEP
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