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1Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology,Jagiellonian University in Krakow, 2Department of Histology,Jagiellonian University Medical College, 3Department of Experimental Hematology, Institute of Zoology and Biomedical Research, Faculty of Biology,Jagiellonian University in Krakow
Chapters
- 00:04Introduction
- 01:03Isolation of Porcine Cumulus-oocyte Complexes (COCs)
- 02:35Encapsulation in Fibrin-alginate Hydrogel Beads
- 04:48Encapsulation in Super-hydrophobic Fluorinated Ethylene Propylene (FEP) Microbioreactors
- 06:17Results: Morphology and Viability of COCs after Encapsulation
- 07:15Conclusion
Apresentados aqui estão dois protocolos para o encapsulamento de oócitos suínos em condições culturais 3D. No primeiro, os complexos cumulus-oocyte (COCs) são encapsulados em contas fibrina-alginato. No segundo, são fechados com partículas fluoradas de propileno de etileno (microbioreatores). Ambos os sistemas garantem condições ideais para manter sua organização 3D.
