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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Modélisation de l’infection par le virus de l’hépatite B dans les cellules non hépatiques 293T-NE-3NRs
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Modélisation de l’infection par le virus de l’hépatite B dans les cellules non hépatiques 293T-NE-3NRs

Article DOI: 10.3791/61414-v • 09:02 min • June 5th, 2020
June 5th, 2020 •
*1,2,3, *1,2,3,4, 1,2,3, 1,2,3,5, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Stem Cell Research Center, Shantou University Medical College, 2The Center for Reproductive Medicine, Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications
* These authors contributed equally

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Ce manuscrit décrit un protocole détaillé pour l’infection par le virus de l’hépatite B (VHB) dans de nouvelles cellules 293T (293T-NE-3NR, exprimant des NTCP humains, HNF4α, RXRα et PPARα) et des cellules hépatiques traditionnelles (PleG2-NE, exprimant le NTCP humain).

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Ce mois-ci dans JoVE Numéro 160 infection par le VHB modèle HepG2.2.15 HepG-NE 293T-NE-3NRs NTCP récepteurs hormonaux nucléaires
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