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Journal / Immunology and Infection / Modelagem da infecção por vírus da hepatite B em…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Modelagem da infecção por vírus da hepatite B em células não hepáticas 293T-NE-3NRs
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Modelagem da infecção por vírus da hepatite B em células não hepáticas 293T-NE-3NRs

Article DOI: 10.3791/61414-v • 09:02 min • June 5th, 2020
June 5th, 2020 •
*1,2,3, *1,2,3,4, 1,2,3, 1,2,3,5, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Stem Cell Research Center, Shantou University Medical College, 2The Center for Reproductive Medicine, Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications
* These authors contributed equally

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Este manuscrito descreve um protocolo detalhado para infecção pelo vírus da Hepatite B (HBV) em novas células 293T (293T-NE-3NRs, expressando NTCP humano, HNF4α, RXRα e PPARα) e células hepáticas tradicionais (HepG2-NE, expressando NTCP humano).

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Este mês em JoVE Edição 160 Infecção por HBV modelo HepG2.2.15 HepG-NE 293T-NE-3NRs NTCP receptores hormonais nucleares
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