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Isolering och kultur chick Ciliary Ganglion nervceller

Article DOI: 10.3791/61431-v • 14:36 min • August 8th, 2020
August 8th, 2020 •
*1, *1, 2, 2, 1,2
1Institute of Biomedicine, Department of Medical Sciences - iBiMED, University of Aveiro, 2CNC - Center for Neuroscience and Cell Biology, University of Coimbra
* These authors contributed equally

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Chick ciliary ganglier (CG) är en del av det parasympatiska nervsystemet. Neuronala kulturer av chick CG nervceller visade sig vara effektiva cellmodeller i studien av nervmuskelinteraktioner. Vi beskriver ett detaljerat protokoll för dissekering, dissociation och in vitro kultur CG nervceller från chick embryon.

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Neurovetenskap Nummer 162 Cellkultur ciliary ganglion kyckling embryo dissekering parasympatiska nervceller neuromuskulära korsningar immuncytokemi fluorescensmikroskopi
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