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经济高效且适应性强的划痕迁移检测
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Biology
A Cost Effective and Adaptable Scratch Migration Assay
Please note that all translations are automatically generated.
Click here for the English version.
经济高效且适应性强的划痕迁移检测
DOI:
10.3791/61527-v
•
08:59 min
•
June 30, 2020
•
Stephanie D. Burr
,
James A Stewart, Jr.
1
Department of BioMolecular Sciences
,
University of Mississippi
Chapters
00:04
Introduction
00:33
Migration Plate Preparation
01:11
Scratch Migration Assay
02:13
Fixation
03:28
Image Analysis
05:20
Migrating Cell Quantification and Migration Area Determination
06:56
Results: Representative Scratch Migration Assay Analyses
08:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们为划痕迁移分析提供了一种经济高效的方法,它提供了一种无需使用设备密集型方法即可确定细胞迁移的新方法。虽然本协议使用了成纤维细胞,但它可以被改编并用于研究其他细胞类型和对细胞迁移的影响。
Tags
Scratch Migration Assay
Cost-effective
Adaptable
Cardiac Fibroblasts
Migration
Scratch
48-well Plate
Low Serum Medium
Microscopy
Imaging Analysis
Paraformaldehyde
Permeabilization
Coomassie Brilliant Blue Staining
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