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JoVE Journal
Biology

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Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera
 

Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera

Article DOI: 10.3791/57691 05:27 min August 8th, 2018
August 8th, 2018

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Summary

Here we describe a procedure to test the cell migration in vitro with an automated optical camera microscope. The scratch assay has been widely used where the closure of the scratch is followed for a set period. The optical microscope enables dependable and cheap detection of cell migration.

Transcript

Tags

Optimized Scratch Assay In Vitro Testing Cell Migration Automated Optical Camera Chronic Wounds Cancer Therapy HaCaT Cells Trypsin Incubation Enzymatic Reaction FBS Concentration Tissue Culture Plate Cell Culture Incubator Confluency Supernatant Replacement Mitomycin C Incubation Wash
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