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Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera

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JoVE Journal Biology

Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera

Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera

DOI:

10.3791/57691-v

•

05:27 min

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August 08, 2018

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Michelle Vang Mouritzen, Håvard Jenssen

¹Department of Science and Environment,Roskilde University

Chapters

00:04Title
00:23HaCaT Monolayer Generation
01:50Migration Stimulation and Optical Camera Setup
03:59Results: Representative Wound Healing Analyses
04:54Conclusion

Summary

Automatic Translation

Here we describe a procedure to test the cell migration in vitro with an automated optical camera microscope. The scratch assay has been widely used where the closure of the scratch is followed for a set period. The optical microscope enables dependable and cheap detection of cell migration.

Tags

Keywords: Cell Migration Scratch Assay Automated Optical Camera HaCaT Cells Trypsin Mitomycin C Wound Healing Cell Proliferation Endothelial Growth Factor

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