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Biochemistry

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JoVE Journal Biochemistry
Aktivering af real-time kompensation i hurtige fotokemiske oxidationer af proteiner til bestemmelse af Protein Topografi Ændringer
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Aktivering af real-time kompensation i hurtige fotokemiske oxidationer af proteiner til bestemmelse af Protein Topografi Ændringer

Article DOI: 10.3791/61580-v • 07:38 min • September 1st, 2020
September 1st, 2020 •
1, 1,2,3
1Department of Biomolecular Sciences, University of Mississippi, 2Department of Chemistry and Biochemistry, University of Mississippi, 3GenNext Technologies, Inc.

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Hurtig fotokemisk oxidation af proteiner er en ny teknik til strukturel karakterisering af proteiner. Forskellige opløsningsmiddeltilsætningsstoffer og ligands har varieret hydroxyl radikale skyllevæske egenskaber. For at sammenligne proteinstrukturen under forskellige forhold kræves der en real-time kompensation af hydroxyl radikaler genereret i reaktionen for at normalisere reaktionsbetingelserne.

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Biokemi Hurtig fotokemisk oxidation af proteiner (FPOP) proteinfodertryk biosimilære kompensation flydende kromatografi-massespektrometri (LC-MS)
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