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Biochemistry

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JoVE Journal Biochemistry
Visualizando dinâmica difusional de nanorods de ouro na membrana celular usando microscopia de campo escuro de nanopartícula única
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Visualizando dinâmica difusional de nanorods de ouro na membrana celular usando microscopia de campo escuro de nanopartícula única

Article DOI: 10.3791/61603-v • 09:09 min • March 5th, 2021
March 5th, 2021 •
1, 1, 1
1Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University

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Aqui, mostramos o uso da microscopia tradicional de campo escuro para monitorar a dinâmica dos nanorods de ouro (AuNRs) na membrana celular. A localização e orientação de AuNRs únicos são detectadas usando ImageJ e MATLAB, e os estados difusivos de AuNRs são caracterizados por uma análise de rastreamento de partículas únicas.

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Bioquímica Edição 169 membrana celular rastreamento de partículas únicas microscopia de campo escuro dinâmica difusa nanorods de ouro
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