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Journal / Biochemistry / Usando Microfluidos e Microscopia de…
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Biochemistry

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JoVE Journal Biochemistry
Usando Microfluidos e Microscopia de Fluorescência para Estudar a Dinâmica de Montagem de Filamentos e Pacotes de Actin Único
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
Journal (Biochemistry)
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
Journal (Biochemistry)
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
 
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Usando Microfluidos e Microscopia de Fluorescência para Estudar a Dinâmica de Montagem de Filamentos e Pacotes de Actin Único

Article DOI: 10.3791/63891-v • 08:02 min • May 5th, 2022
May 5th, 2022 •
1, 1, 1, 1, 1
1Université Paris Cité, CNRS, Institut Jacques Monod

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Apresentamos protocolos para ensaios microfluidos de filamento de actina simples, em combinação com microscopia de fluorescência, que permitem monitorar com precisão filamentos de actina individuais em tempo real, expondo-os sequencialmente a diferentes soluções proteicas.

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Bioquímica Edição 183
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