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Biochemistry

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Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
 

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Article DOI: 10.3791/61680-v
September 20th, 2020

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Summary September 20th, 2020

Single molecule RNA fluorescence in situ hybridization (smFISH) is a method to accurately quantify levels and localization of specific RNAs at the single cell level. Here, we report our validated lab protocols for wet-bench processing, imaging and image analysis for single cell quantification of specific RNAs.

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