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使用刺激拉曼散射显微镜对 卡诺哈布迪炎电子人 中的脂质存储动力学进行无标签成像

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Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Please note that all translations are automatically generated. Click here for the English version.

使用刺激拉曼散射显微镜对卡诺哈布迪炎电子人中的脂质存储动力学进行无标签成像

DOI:

10.3791/61870-v

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10:59 min

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May 28, 2021

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Ayse Sena Mutlu, Tao Chen, Dinghuan Deng, Meng C. Wang^2,3

¹Huffington Center on Aging,Baylor College of Medicine, ²Department of Molecular and Human Genetics,Baylor College of Medicine, ³Howard Hughes Medical Institute,Baylor College of Medicine

Chapters

00:05Introduction
00:51Stimulated Raman Scattering (SRS) Microscopy Instrument Setup
03:53Imaging Condition Optimization
05:21C. elegans Sample Preparation
06:16Image Acquisition and Analysis
07:26Image Analysis
08:37Results: Representative Effects of Insulin Signaling on Lipid Storage in C. elegans
10:03Conclusion

Summary

Automatic Translation

刺激拉曼散射（SRS）显微镜允许选择性，无标签成像的特定化学莫伊蒂，它已被有效地用于图像脂质分子在体内。在这里，我们简要介绍了SRS显微镜原理，并描述了用于 在卡诺哈布迪炎电子群中成像脂质储存的方法。

Tags

Stimulated Raman Scattering Microscopy Label-free Imaging Lipid Storage Caenorhabditis Elegans Laser Alignment Beam Expander Relay Mirrors Periscope Power Meter Objective Alignment Fluorescence Target Alignment Cap

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