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使用刺激拉曼散射显微镜对 卡诺哈布迪炎电子人 中的脂质存储动力学进行无标签成像
JoVE Journal
Biology
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JoVE Journal Biology
Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
DOI:

10:59 min

May 28, 2021

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Chapters

  • 00:05Introduction
  • 00:51Stimulated Raman Scattering (SRS) Microscopy Instrument Setup
  • 03:53Imaging Condition Optimization
  • 05:21C. elegans Sample Preparation
  • 06:16Image Acquisition and Analysis
  • 07:26Image Analysis
  • 08:37Results: Representative Effects of Insulin Signaling on Lipid Storage in C. elegans
  • 10:03Conclusion

Summary

Automatic Translation

刺激拉曼散射(SRS)显微镜允许选择性,无标签成像的特定化学莫伊蒂,它已被有效地用于图像脂质分子在体内。在这里,我们简要介绍了SRS显微镜原理,并描述了用于 在卡诺哈布迪炎电子群中成像脂质储存的方法。

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