Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Ayse Sena Mutlu; Tao Chen; Dinghuan Deng; Meng  C. Wang

doi:10.3791/61870

/

Label-Free Imaging af Lipid Storage Dynamics i Caenorhabditis elegans ved hjælp af stimuleret Raman Scattering Mikroskopi

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Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Label-Free Imaging af Lipid Storage Dynamics i Caenorhabditis elegans ved hjælp af stimuleret Raman Scattering Mikroskopi

DOI:

10.3791/61870-v

•

10:59 min

•

May 28, 2021

•

Ayse Sena Mutlu, Tao Chen, Dinghuan Deng, Meng C. Wang^2,3

¹Huffington Center on Aging,Baylor College of Medicine, ²Department of Molecular and Human Genetics,Baylor College of Medicine, ³Howard Hughes Medical Institute,Baylor College of Medicine

Chapters

00:05Introduction
00:51Stimulated Raman Scattering (SRS) Microscopy Instrument Setup
03:53Imaging Condition Optimization
05:21C. elegans Sample Preparation
06:16Image Acquisition and Analysis
07:26Image Analysis
08:37Results: Representative Effects of Insulin Signaling on Lipid Storage in C. elegans
10:03Conclusion

Summary

Automatic Translation

Stimuleret Raman spredning (SRS) mikroskopi giver mulighed for selektiv, etiket-fri billeddannelse af specifikke kemiske moieties og det er blevet effektivt ansat til at billedet lipid molekyler in vivo. Her giver vi en kort introduktion til princippet om SRS mikroskopi og beskrive metoder til dets anvendelse i billedbehandling lipid opbevaring i Caenorhabditis elegans.

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