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使用刺激拉曼散射显微镜对
卡诺哈布迪炎电子人
中的脂质存储动力学进行无标签成像
JoVE Journal
Biology
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JoVE Journal
Biology
Label-Free Imaging of Lipid Storage Dynamics in
Caenorhabditis elegans
using Stimulated Raman Scattering Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用刺激拉曼散射显微镜对
卡诺哈布迪炎电子人
中的脂质存储动力学进行无标签成像
DOI:
10.3791/61870-v
•
10:59 min
•
May 28, 2021
•
Ayse Sena Mutlu
,
Tao Chen
,
Dinghuan Deng
,
Meng C. Wang
2,3
1
Huffington Center on Aging
,
Baylor College of Medicine
,
2
Department of Molecular and Human Genetics
,
Baylor College of Medicine
,
3
Howard Hughes Medical Institute
,
Baylor College of Medicine
Chapters
00:05
Introduction
00:51
Stimulated Raman Scattering (SRS) Microscopy Instrument Setup
03:53
Imaging Condition Optimization
05:21
C. elegans
Sample Preparation
06:16
Image Acquisition and Analysis
07:26
Image Analysis
08:37
Results: Representative Effects of Insulin Signaling on Lipid Storage in
C. elegans
10:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
刺激拉曼散射(SRS)显微镜允许选择性,无标签成像的特定化学莫伊蒂,它已被有效地用于图像脂质分子在体内。在这里,我们简要介绍了SRS显微镜原理,并描述了用于
在卡诺哈布迪炎电子群
中成像脂质储存的方法。
Tags
Stimulated Raman Scattering Microscopy
Label-free Imaging
Lipid Storage
Caenorhabditis Elegans
Laser Alignment
Beam Expander
Relay Mirrors
Periscope
Power Meter
Objective Alignment
Fluorescence Target Alignment Cap
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无标签
原位
成像在植物细胞壁的木质化
差分成像生物结构与双共振相干反斯托克斯拉曼散射(CARS)
分离和<em在体外激活
线虫
精子
时间推移显微镜早期胚胎发育
线虫
基本的<em>线虫</em>方法:同步和观察
前列腺素的提取与分析<em>线虫</em
Using
Caenorhabditis elegans
as a Model System to Study Protein Homeostasis in a Multicellular Organism
尼罗红与油红 O 染色法测定
秀丽线虫
的脂质丰度及脂质分布评价
通过总内部反射荧光显微镜与数量和亮度分析相结合,活细胞中细胞表面受体的显微化动力学
使用
卡诺哈布迪炎埃莱甘斯
筛选组织特异性查佩龙相互作用
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