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Journal / Bioengineering / Costruire un sistema di illuminazione semplice e…
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Bioengineering

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JoVE Journal Bioengineering
Costruire un sistema di illuminazione semplice e versatile per esperimenti optogenetici
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Costruire un sistema di illuminazione semplice e versatile per esperimenti optogenetici

Article DOI: 10.3791/61914-v • 06:41 min • January 12th, 2021
January 12th, 2021 •
1, 2, 1, 1, 1, 3, 1, 1, 4, 1, 1
1Department of Bioengineering, University of California, San Diego, 2University of California, San Diego, 3Department of Mathematical Computational, and Systems biology, University of California, Irvine, 4Department of Biomedical Engineering, Duke University

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Questo protocollo descrive come eseguire esperimenti optogenetici per controllare l'espressione genica con luce rossa e rossa lontana utilizzando PhyB e PIF3. Sono incluse istruzioni dettagliate per la costruzione di un sistema di illuminazione semplice e flessibile, che consente il controllo dell'espressione genica o di altre optogenetiche con un computer.

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Bioingegneria Numero 167 optogenetica PhyB fitocromo LOV CRY2 nMag-pMag microcontrollore LED elettronica DIY LED illuminazione
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