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Ett ekonomiskt och mångsidigt proteinreningssystem med hög genomströmning med en plattadapter med flera kolumner
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
DOI:

10:08 min

May 21, 2021

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Chapters

  • 00:00Introduction
  • 00:58Ni-NTA Chromatography- Purification of 24 Different Proteins: Preparing the Purification Set-up
  • 03:00Ni-NTA Chromatography- Purification of 24 Different Proteins: Loading, Washing and Eluting
  • 04:47Ion Exchange- Simultaneous Purification of 6 Different Proteins: Preparing the Purification Set-up
  • 06:15Ion Exchange- Simultaneous Purification of 6 Different Proteins: Loading, Washing and Eluting
  • 08:25Results: Representative UV Spectroscopy and SDS PAGE Analyses

Summary

Automatic Translation

En flerkolonnsplattaadapter gör det möjligt att koppla kromatografikolonner till flerbrunnsuppsamlingsplattor för parallell affinitet eller jonutbytesrening som ger en ekonomisk proteinreningsmetod med hög genomströmning. Det kan användas under gravitation eller vakuum som ger milligram mängder protein via prisvärd instrumentering.

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