Journal
/
/
Et økonomisk og alsidigt proteinrensningssystem med høj gennemløb ved hjælp af en pladeadapter med flere kolonner
JoVE Journal
Biochemistry
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
DOI:

10:08 min

May 21, 2021

, , , , ,

Chapters

  • 00:00Introduction
  • 00:58Ni-NTA Chromatography- Purification of 24 Different Proteins: Preparing the Purification Set-up
  • 03:00Ni-NTA Chromatography- Purification of 24 Different Proteins: Loading, Washing and Eluting
  • 04:47Ion Exchange- Simultaneous Purification of 6 Different Proteins: Preparing the Purification Set-up
  • 06:15Ion Exchange- Simultaneous Purification of 6 Different Proteins: Loading, Washing and Eluting
  • 08:25Results: Representative UV Spectroscopy and SDS PAGE Analyses

Summary

Automatic Translation

En multi-kolonne plade adapter gør det muligt kromatografi kolonner, der skal interfaces med multi-well indsamling plader til parallel affinitet eller ion udveksling rensning giver en økonomisk høj gennemløb protein rensning metode. Det kan bruges under tyngdekraften eller vakuum giver milligram mængder af protein via overkommelig instrumentering.

Related Videos

Read Article